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2× hybridization buffer helicos rna sequencing kit  (Helicos Inc)

 
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    Structured Review

    Helicos Inc 2× hybridization buffer helicos rna sequencing kit
    DRS sample preparation. RNA species which contain a 3' poly-A tail require 3' end blocking as described. Other RNA species are enzymatically 3' polyadenylated and 3' blocked. The blocking step is performed to prevent “downward” nucleotide additions to the 3' end of the template during the sequencing process (details of the sequencing strategy and chemistry have been described previously (32)). Polyadenylated RNA is captured on the sequencing flow cell surfaces coated with poly(dT) oligonucleotides through <t>hybridization.</t> A “fill” step is performed with dTTP and polymerase, and then the templates are “locked” in position with fluorescently labeled proprietary Virtual Terminator™ (VT)-A, -C and -G sequencing nucleotide analogs. VT analogs are nucleotides used for sequencing, containing a fluores-cent dye and chemically cleavable groups that prevent the addition of another nucleotide. These “fill and lock” steps correct for any misalignments that may be present in poly-A/T duplexes, and ensure that the sequencing starts in the template rather than the poly-A tail.
    2× Hybridization Buffer Helicos Rna Sequencing Kit, supplied by Helicos Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2%C3%97+hybridization+buffer+helicos+rna+sequencing+kit/pmc03272358-114-8-17?v=Helicos+Inc
    Average 90 stars, based on 1 article reviews
    2× hybridization buffer helicos rna sequencing kit - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing"

    Article Title: Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing

    Journal: Methods in Molecular Biology (Clifton, N.j.)

    doi: 10.1007/978-1-61779-089-8_4

    DRS sample preparation. RNA species which contain a 3' poly-A tail require 3' end blocking as described. Other RNA species are enzymatically 3' polyadenylated and 3' blocked. The blocking step is performed to prevent “downward” nucleotide additions to the 3' end of the template during the sequencing process (details of the sequencing strategy and chemistry have been described previously (32)). Polyadenylated RNA is captured on the sequencing flow cell surfaces coated with poly(dT) oligonucleotides through hybridization. A “fill” step is performed with dTTP and polymerase, and then the templates are “locked” in position with fluorescently labeled proprietary Virtual Terminator™ (VT)-A, -C and -G sequencing nucleotide analogs. VT analogs are nucleotides used for sequencing, containing a fluores-cent dye and chemically cleavable groups that prevent the addition of another nucleotide. These “fill and lock” steps correct for any misalignments that may be present in poly-A/T duplexes, and ensure that the sequencing starts in the template rather than the poly-A tail.
    Figure Legend Snippet: DRS sample preparation. RNA species which contain a 3' poly-A tail require 3' end blocking as described. Other RNA species are enzymatically 3' polyadenylated and 3' blocked. The blocking step is performed to prevent “downward” nucleotide additions to the 3' end of the template during the sequencing process (details of the sequencing strategy and chemistry have been described previously (32)). Polyadenylated RNA is captured on the sequencing flow cell surfaces coated with poly(dT) oligonucleotides through hybridization. A “fill” step is performed with dTTP and polymerase, and then the templates are “locked” in position with fluorescently labeled proprietary Virtual Terminator™ (VT)-A, -C and -G sequencing nucleotide analogs. VT analogs are nucleotides used for sequencing, containing a fluores-cent dye and chemically cleavable groups that prevent the addition of another nucleotide. These “fill and lock” steps correct for any misalignments that may be present in poly-A/T duplexes, and ensure that the sequencing starts in the template rather than the poly-A tail.

    Techniques Used: Sample Prep, Blocking Assay, Sequencing, Hybridization, Labeling



    Similar Products

    2× hybridization buffer helicos rna sequencing kit  (Helicos Inc)
    90
    Helicos Inc 2× hybridization buffer helicos rna sequencing kit
    DRS sample preparation. RNA species which contain a 3' poly-A tail require 3' end blocking as described. Other RNA species are enzymatically 3' polyadenylated and 3' blocked. The blocking step is performed to prevent “downward” nucleotide additions to the 3' end of the template during the sequencing process (details of the sequencing strategy and chemistry have been described previously (32)). Polyadenylated RNA is captured on the sequencing flow cell surfaces coated with poly(dT) oligonucleotides through <t>hybridization.</t> A “fill” step is performed with dTTP and polymerase, and then the templates are “locked” in position with fluorescently labeled proprietary Virtual Terminator™ (VT)-A, -C and -G sequencing nucleotide analogs. VT analogs are nucleotides used for sequencing, containing a fluores-cent dye and chemically cleavable groups that prevent the addition of another nucleotide. These “fill and lock” steps correct for any misalignments that may be present in poly-A/T duplexes, and ensure that the sequencing starts in the template rather than the poly-A tail.
    2× Hybridization Buffer Helicos Rna Sequencing Kit, supplied by Helicos Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2%C3%97+hybridization+buffer+helicos+rna+sequencing+kit/pmc03272358-114-8-17?v=Helicos+Inc
    Average 90 stars, based on 1 article reviews
    2× hybridization buffer helicos rna sequencing kit - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    DRS sample preparation. RNA species which contain a 3' poly-A tail require 3' end blocking as described. Other RNA species are enzymatically 3' polyadenylated and 3' blocked. The blocking step is performed to prevent “downward” nucleotide additions to the 3' end of the template during the sequencing process (details of the sequencing strategy and chemistry have been described previously (32)). Polyadenylated RNA is captured on the sequencing flow cell surfaces coated with poly(dT) oligonucleotides through hybridization. A “fill” step is performed with dTTP and polymerase, and then the templates are “locked” in position with fluorescently labeled proprietary Virtual Terminator™ (VT)-A, -C and -G sequencing nucleotide analogs. VT analogs are nucleotides used for sequencing, containing a fluores-cent dye and chemically cleavable groups that prevent the addition of another nucleotide. These “fill and lock” steps correct for any misalignments that may be present in poly-A/T duplexes, and ensure that the sequencing starts in the template rather than the poly-A tail.

    Journal: Methods in Molecular Biology (Clifton, N.j.)

    Article Title: Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing

    doi: 10.1007/978-1-61779-089-8_4

    Figure Lengend Snippet: DRS sample preparation. RNA species which contain a 3' poly-A tail require 3' end blocking as described. Other RNA species are enzymatically 3' polyadenylated and 3' blocked. The blocking step is performed to prevent “downward” nucleotide additions to the 3' end of the template during the sequencing process (details of the sequencing strategy and chemistry have been described previously (32)). Polyadenylated RNA is captured on the sequencing flow cell surfaces coated with poly(dT) oligonucleotides through hybridization. A “fill” step is performed with dTTP and polymerase, and then the templates are “locked” in position with fluorescently labeled proprietary Virtual Terminator™ (VT)-A, -C and -G sequencing nucleotide analogs. VT analogs are nucleotides used for sequencing, containing a fluores-cent dye and chemically cleavable groups that prevent the addition of another nucleotide. These “fill and lock” steps correct for any misalignments that may be present in poly-A/T duplexes, and ensure that the sequencing starts in the template rather than the poly-A tail.

    Article Snippet: The RNA samples are mixed 50:50 with 2× hybridization buffer provided in the Helicos RNA Sequencing Kit (Helicos BioSciences Corporation).

    Techniques: Sample Prep, Blocking Assay, Sequencing, Hybridization, Labeling